CST, 9231S, Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody

Polyclonal Antibody for studying MKK3 (Ser218) phosphate/MKK6 (Ser207) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody (CST #9231) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. This antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography. Background MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5). Alternate Names Dual specificity mitogen-activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 3; MAP kinase kinase 6; MAP2K3; MAP2K6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; MEK3; MEK6; mitogen-activated protein kinase kinase 3; mitogen-activated protein kinase kinase 6; MKK3; MKK6; MP2K3; MP2K6; PRKMK3; PRKMK6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPK kinase 3; SAPKK-2; SAPKK-3; SAPKK2; SAPKK3; SKK2; SKK3; Stress-activated protein kinase kinase 2; Stress-activated protein kinase kinase 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Phospho-MKK3. 41 Phospho-MKK6. SOURCE: Rabbit