CST, 92405S, SUN2 (F6Y2S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SUN2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SUN2 (F6Y2S) Rabbit Monoclonal Antibody (CST #92405) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SUN2 (F6Y2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SUN2 protein. This antibody does not cross-react with SUN1 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala93 of human SUN2 protein. Background The Sad1 and UNC domain proteins (SUN1 and SUN2) are nuclear envelope-associated proteins with similar C-terminal domains. SUN1 and SUN2 are part of the linker of nucleoskeleton and cytoskeleton (LINC) complex, which spans the nuclear envelope and binds to lamin A, providing key structural support (1-3). Furthermore, SUN1 and SUN2 bind to the Klarsicht, ANC-1, Syne Homology (KASH) domains of various proteins, such as the nespirins, to tether them to the outer nuclear membrane (3,4). While SUN1 and SUN2 have some redundancy, their localization differs slightly, with SUN1 being more concentrated at nuclear pore complexes (5). SUN1 and SUN2 are critical for nuclear positioning in many cellular processes, including muscle and neuronal development, meiosis, and viral response (6-10). SUN proteins have also been shown to be required for mRNA export by interacting with various hnRNP proteins (11). Alternate Names FRIGG; KIAA0668; MGC133055; MGC133056; nuclear envelope protein; Protein unc-84 homolog B; Rab5-interacting protein; RAB5IP; Sad1 and UNC84 domain containing 2; Sad1 unc-84 domain protein 2; Sad1/unc-84 protein-like 2; SUN domain-containing protein 2; SUN2; unc-84 homolog B; UNC84B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG