CST, 92427T, MAP4K3 (D1L4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAP4K3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MAP4K3 (D1L4G) Rabbit Monoclonal Antibody (CST #92427) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAP4K3 (D1L4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAP4K3 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu452 of human MAP4K3 protein. Background The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by growth factors and nutrients including amino acids (1,2). MAP4K3 is a mediator between nutrient signal and mTORC1 (1). Research studies suggest that amino acid sufficiency leads to the phosphorylation of Ser170 on MAP4K3, which activates mTORC1 (3). Alternate Names germinal center kinase-like kinase; Germinal center kinase-related protein kinase; GLK; KHS2; M4K3; MAP4K3; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; MEKKK 3; MEKKK3; Mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 101 Source/Isotype: Rabbit IgG