CST, 9242S, IkappaB alpha Antibody

Polyclonal Antibody for studying IkB-a. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IkappaB alpha Antibody (CST #9242) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IkappaB alpha Antibody detects endogenous levels of total IκBα protein. Under some experimental conditions this antibody binds preferentially to IκBα that is not phosphorylated at Ser32/Ser36. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Dog, Pig Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Arg29 of human IκBα. Antibodies are purified by protein A and peptide affinity chromatography. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). Alternate Names EDAID2; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Specification REACTIVITY: H M R Mk B Dg Pg SENSITIVITY: Endogenous MW (kDa): 39 SOURCE: Rabbit