CST, 92438S, Phospho-AAK1 (Ser637/Thr640) Antibody

Polyclonal Antibody for studying AAK1 (Ser637) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-AAK1 (Ser637/Thr640) Antibody (CST #92438) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-AAK1 (Ser637/Thr640) Antibody recognizes endogenous levels of AAK1 protein only when phosphorylated at Ser637, Thr640, and Ser637/Thr640. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser637/Thr640 of human AAK1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Adaptor-associated kinase 1 (AAK1) is a member of the Ark1/Prk1 family of serine/threonine kinases (1). AAK1 is enriched in synaptosomal preparations and modulates clathrin-dependent endocytosis, a process that is important in synaptic vesicle recycling and receptor-mediated endocytosis. AAK1, together with clathrin and clathrin-adaptor protein AP-2, forms a signaling complex at the cell membrane. AAK1-dependent phosphorylation of the mu-2 subunit of AP-2 enhances endocytosis efficiency (2,3). AAK1 is known to promote neuregulin/ErbB4 internalization to regulate neurotrophic signaling. Inhibition of AAK1 activity promotes cell surface expression of neuregulin/ErbB4, cell-bound neurotrophic factors that are implicated in brain development and synaptic plasticity (4). Alternate Names AAK1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; KIAA1048; MGC138170; MGC164568; MGC164570 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 142 SOURCE: Rabbit