CST, 9243S, NF-kappaB Control Cell Extracts

Cell Extract Kit for studying IkB-a/IKK-beta/IKK-alpha in the research area. Product Usage Information Boil for 3 minutes prior to use. Load 20 µl of untreated and hTNF-α treated NF-kappaB Control Cell Extracts per lane. Storage Supplied in SDS Sample Buffer : 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Store at -20°C or -80°C for long-term storage. Background Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). Alternate Names cell extracts; control extracts; extracts; hela; ikappab; ikbb; ikk; IκB-α; iκbα; nf-kappab; nf-kb; nfkb; rel; tnf