CST, 9246L, Phospho-IkappaB alpha (Ser32/36) (5A5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying IkB-a (Ser32/Ser36) phosphate. Validated for Western Blotting,Simple Western™. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-IkappaB alpha (Ser32/36) (5A5) Mouse Monoclonal Antibody (CST #9246) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37650 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IkappaB alpha (Ser32/36) (5A5) Mouse Monoclonal Antibody detects endogenous levels of IκBα only when phosphorylated at Ser32/36. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). Alternate Names EDAID2; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Mouse IgG1