CST, 92470S, PLXND1 Antibody

Polyclonal Antibody for studying PLXND1. Validated for Western Blotting. Highly specific and rigorously validated in-house, PLXND1 Antibody (CST #92470) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PLXND1 Antibody recognizes endogenous levels of total PLXND1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1665 of human PLXND1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background PLXND1 (PlexinD1) is a type I transmembrane receptor for semaphorin (SEMA) family signaling molecules (1). PLXND1 has an extracellular SEMA binding domain, and a cytoplasmic tail containing RasGAP motifs and a RhoGTPase-binding domain. Upon ligand binding, PLXND1 undergoes conformational change and acquires GAP activity that inactivates downstream Rac/Ras signaling, leading to focal adhesion destabilization (2). The PLXND1 signaling pathway plays important roles in neuronal synapse formation, vascular branching, and thymocyte migration (2-4). Increased expression of PLXND1 is positively correlated with tumor stages in multiple cancer types (6). This is supported by gene knockdown experiments that suggest that SEMA/PLXND1 signaling may contribute metastatic progression (7-8). Alternate Names KIAA0620; MGC75353; PLEXD1; plexin D1; Plexin-D1; PLXD1; PLXND1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 250 SOURCE: Rabbit