CST, 92511S, Human CD3 epsilon Activating (OKT3) Mouse Monoclonal Antibody (Low Endotoxin, Azide-free)

Monoclonal Antibody for studying CD3epsilon. Validated for Functional. Highly specific and rigorously validated in-house, Human CD3 epsilon Activating (OKT3) Mouse Monoclonal Antibody (Low Endotoxin, Azide-free) (CST #92511) is ready to ship. Formulation Supplied in 10 mM NaH2PO4, 150 mM NaCl, pH7.2, Low Endotoxin, Azide-free. Storage Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Protocol Available protocols: Functional Specificity / Sensitivity Human CD3 epsilon Activating (OKT3) Mouse Monoclonal Antibody (Low Endotoxin, Azide-free) recognizes endogenous levels of CD3ε protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced against human PBMC cells and purified via affinity chromatography. Background When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5). Alternate Names CD3-epsilon; CD3E; CD3E antigen, epsilon polypeptide; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; FLJ18683; IMD18; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG2a kappa