CST, 92579S, POU2F3 Antibody

Polyclonal Antibody for studying OCT11/POU2F3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, POU2F3 Antibody (CST #92579) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity POU2F3 Antibody recognizes endogenous levels of total POU2F3 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu160 of human POU2F3 protein. Antibodies are purified by peptide affinity chromatography. Background POU2F3 (also known as OCT-11 and SKN-1) belongs to the OCT family of octamer motif-binding transcription factors, members of which play varied, diverse roles in animal development. POU2F3 is a lineage-defining transcription factor, responsible for driving differentiation of specialized chemosensory cells in the gastrointestinal and respiratory tracts (1-3). Tuft cells are a subtype of chemosensory cell, defined by POU2F3 expression, and which are hypothesized to be the origin of a distinct, non-neuroendocrine subtype of small cell lung cancer (SCLC) known as tuft cell-like SCLC (4). Consistent with its lineage-defining role in tuft cell development, POU2F3 was identified through a Crispr/Cas9 screen as a master regulator of tuft cell-like SCLC (5). Alternate Names Epoc-1; FLJ40063; MGC126698; Oct-11; OCT11; Octamer-binding protein 11; Octamer-binding transcription factor 11; OTF-11; OTF11; PLA-1; PLA1; PO2F3; POU class 2 homeobox 3; POU domain transcription factor OCT11a; POU domain, class 2, transcription factor 3; POU transcription factor; POU2F3; Skn-1a; Transcription factor PLA-1; Transcription factor Skn-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-60 SOURCE: Rabbit