CST, 9258T, JNK2 (56G8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JNK2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, JNK2 (56G8) Rabbit Monoclonal Antibody (CST #9258) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 15676 . Protocol Available protocols: Western Blotting Specificity / Sensitivity JNK2 (56G8) Rabbit Monoclonal Antibody detects endogenous levels of total JNK2 protein. This antibody does not recognize JNK1 or JNK3 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Mink Source / Purification Monoclonal antibody is produced by immunizing animals with a human JNK2/MBP fusion protein. Background The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). Alternate Names c-Jun kinase 2; c-Jun N-terminal kinase 2; JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; Jun kinase; MAP kinase 9; MAPK 9; MAPK9; Mitogen-activated protein kinase 9; mitogen-activated protein kinase 9 isoform JNK2 alpha2; MK09; p54a; p54aSAPK; PRKM9; SAPK; SAPK1A; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2 Specification REACTIVITY: H M R Hm Mk Mi SENSITIVITY: Endogenous MW (kDa): 46, 54 Source/Isotype: Rabbit