CST, 92649S, SDHB (E3H9Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SDHB. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, SDHB (E3H9Z) Rabbit Monoclonal Antibody (CST #92649) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SDHB (E3H9Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total SDHB protein. This antibody detects a 120 kDa protein of unknown identity in some cell extracts. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly208 of human SDHB protein. Background Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington's disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas (4,5). Alternate Names CWS2; DHSB; FLJ92337; Ip; Iron-sulfur subunit of complex II; PGL4; SDH; SDH1; SDH2; SDHB; SDHIP; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex iron sulfur subunit B; succinate dehydrogenase complex, subunit B, iron sulfur (Ip) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG