CST, 92654S, NOTUM (F1E9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NOTUM. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, NOTUM (F1E9C) Rabbit Monoclonal Antibody (CST #92654) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NOTUM (F1E9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total NOTUM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His422 of human NOTUM protein. Background NOTUM is an extracellular carboxylesterase and a key negative regulator of the Wnt signaling pathway. The palmitoleoylation of Wnt is required for its binding to Frizzled receptors, which is essential for Wnt signaling activation. NOTUM removes the palmitoleate moiety on Wnt ligands to inactivate Wnt signaling (1,2). The function of NOTUM is dependent on glypicans, which bind both NOTUM and Wnt to co-localize them at the cell surface. NOTUM is expressed in the liver (3), bone (4), epithelial cells in the intestine (5), and brain (6) to regulate tissue formation and function. Biological aging may result from increased NOTUM expression, while inhibition of NOTUM may rejuvenate stem cells to promote tissue regeneration (5). In colorectal cancer and gastric cancer, aberrant high NOTUM expression promotes proliferation and metastasis. Knockdown or inhibition of NOTUM activity suppresses its cancer-promoting effects (7-9). Inhibition of NOTUM activity may represent a new approach to treat disease where aberrant NOTUM activity has been identified as the underlying cause (10). Alternate Names [Wnt protein] O-palmitoleoyl-L-serine hydrolase; hNOTUM; NOTUM; notum pectinacetylesterase homolog; notum pectinacetylesterase homolog (Drosophila); notum, palmitoleoyl-protein carboxylesterase; Palmitoleoyl-protein carboxylesterase NOTUM; Protein notum homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55,62 Source/Isotype: Rabbit IgG