CST, 92658T, ATP5O (E7F4U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATP5O. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ATP5O (E7F4U) Rabbit Monoclonal Antibody (CST #92658) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ATP5O (E7F4U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATP5O protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATP5O protein. Background ATP synthase subunit O (ATP5O, OSCP, or ATP5PO) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. ATP synthase is composed of a soluble catalytic core, F , and a membrane-spanning proton (H ) channel, F . ATP5O is part of the peripheral stalk linking the two structural domains. The ATP5O N-terminus directly interacts with subunit F , while the C-terminus directly interacts with F and is critical for the oligomycin sensitivity of the H channel (1,2). The expression of ATP5O is elevated in cancer-associated fibroblasts (CAFs) compared to normal fibroblasts in oral squamous cell carcinoma, reflecting the elevated oxidative phosphorylation requirements for ATP generation in these cells (3). Recent studies have shown elevated ATP5O as a biomarker for prostate and gastric cancers (4,5). Conversely, direct binding of claudin-10 (CLDN10) to ATP5O in the outer mitochondrial membrane increases ATP5O expression and acetylation, leading to disrupted mitochondria and reduced metastasis in clear cell renal cell carcinoma (ccRCC) (6). Alternate Names ATP synthase peripheral stalk subunit OSCP; ATP synthase subunit O, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; ATP5O; ATP5PO; ATPO; HMC08D05; human ATP synthase OSCP subunit; mitochondrial ATP synthase, O subunit; Oligomycin sensitivity conferral protein; oligomycin sensitivity conferral protein oscp-like protein; oligomycin sensitivity conferring protein; OSCP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG