CST, 92728SF, Prostate Specific Membrane Antigen (D7I8E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PSMA. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Prostate Specific Membrane Antigen (D7I8E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #92728) is ready to ship. Product Usage Information This product is the carrier free version of product #12815. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12815 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Prostate Specific Membrane Antigen (D7I8E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total prostate specific membrane antigen. An SDS resistant dimer at approximately 200 kDa can be detected depending on sample preparation conditions. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of human prostate specific membrane antigen protein. Background Prostate specific membrane antigen (PSMA, also known as FOLH1), a type II transmembrane protein of the M28 family, has both folate hydrolase and N-acetylated-alpha-linked acidic dipeptidase activity. PSMA was originally identified in the LNCaP cell line, which was derived from a prostate adenocarcinoma lymph node metastasis (1,2). PSMA is an established prostate cancer marker (3); however, it is expressed in other tissues, including kidney, liver, and urinary bladder (4), and it is associated with tumor neovasculature (5) as well. Research studies suggest that PSMA is both a potential diagnostic readout and therapeutic target (6-8). Alternate Names Cell growth-inhibiting gene 27 protein; cell growth-inhibiting protein 27; FGCP; folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1; FOLH; FOLH1; Folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; glutamate carboxylase II; Glutamate carboxypeptidase 2; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mGCP; N-acetylated alpha-linked acidic dipeptidase 1; N-acetylated-alpha-linked acidic dipeptidase I; NAALAD1; NAALAdase; NAALADase I; prostate specific membrane antigen variant F; Prostate-specific membrane antigen; PSM; PSMA; Pteroylpoly-gamma-glutamate carboxypeptidase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG