CST, 92761SF, SS18-SSX (E9X9V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SS18/SSX1 fusion. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, SS18-SSX (E9X9V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #92761) is ready to ship. Product Usage Information This product is the carrier-free version of product #72364. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product #www.cellsignal.com/products/primary-antibodies/ss18-ssx-e9x9v-xp-rabbit-mab/72364?site-search-type=Products&N=4294956287&Ntt=ss18&fromPage=plp">72364. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SS18-SSX (E9X9V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SS18-SSX fusion protein. This antibody does not cross-react with wild-type SS18 or SSX proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the fusion site of human SS18-SSX protein. Background SS18 is a protein that has been shown to be a part of the SWI/SNF complex (1, 2). The SS18-SSX fusion proteins are a result of in-frame fusions that fuse the gene on chromosome 18 with X chromosome genes , , and to a lesser extent (3). Human synovial sarcoma (SS) accounts for 8-10% of all soft tissue malignancies and 95% of these malignancies express the recurrent translocation of the gene on chromosome 18 (3). The N-terminal SNH domain (SYT N-terminal homology domain) of the SS18 protein interacts with SWI/SNF chromatin remodeling complexes via the N terminal region of BRM and BRG1 subunits (4). Studies of the SS18-SSX fusion in SS suggest that endogenous SS18 competes with the mutant SS18-SSX fusion for occupancy in the SWI/SNF complexes resulting in the displacement of SNF5 (BAF47) subunit. Displacement of the SNF5 subunit results in altered function of the SWI/SNF complex that leads to deregulated expression of genes such as in SS (3). Alternate Names Fusion protein SYT-SSX1; SS18/SSX1 fusion; SSX1/SS18 fusion; SSX1/SSXT fusion; SSXT/SSX1 fusion; SYT-SSX1; SYT/SSX1 fusion Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65, 75 Source/Isotype: Rabbit IgG