CST, 92839S, PIKFYVE (E4X3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PIKFYVE. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PIKFYVE (E4X3R) Rabbit Monoclonal Antibody (CST #92839) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PIKFYVE (E4X3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PIKFYVE protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1653 of human PIKFYVE protein. Background PIKFYVE is a lipid kinase that phosphorylates position 5 of PIP (phosphatidylinositol) or PI(3)P (phosphatidylinositol 3-phosphate) to form PI5P or PI(3,5)P2 (1,2). The normal cellular levels of the latter two lipids are important in maintaining the functional responses of endosomes, lysosomes, and autophagosomes, as well as the regulation of cell cycle, stress response, T cell activation, and chromatin remodeling (3,4). PIKFYVE functions within a regulatory complex including the PIKFYVE kinase, a scaffold protein Vac14, and a lipid phosphatase Fig4 (Sac) (5,6). The complex localizes to endosomes and lysosomes, regulating endosome-to-TGN retrograde transport, lysosome fission, and lysosomal related autophagy (7-10). Targeted disruption of the gene is embryonic lethal, indicating the vital role of the kinase in early embryonic development (11). Inhibition of PIKFYVE disrupts lysosome function in autophagy and can selectively kill certain cancer cells (12,13). PIKFYVE inhibition also potentiates immune response to checkpoint blockade in the tumor microenvironment (14). Alternate Names 1-phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol-3-phosphate 5-kinase; 1-phosphatidylinositol-4-phosphate 5-kinase; CFD; epididymis luminal protein 37; FAB1; FLJ37746; FYV1; FYVE finger-containing phosphoinositide kinase; HEL37; KIAA0981; MGC40423; Phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase type III; Phosphatidylinositol-3-phosphate 5-kinase; Phosphatidylinositol-3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; phosphoinositide kinase, FYVE finger containing; phosphoinositide kinase, FYVE-type zinc finger containing; PIKFYVE; PIP5K; PIP5K3; PIPkin-III; PtdIns(4)P-5-kinase; Type III PIP kinase; ZFYVE29; zinc finger, FYVE domain containing 29 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 250 Source/Isotype: Rabbit IgG