CST, 92879S, CPSF6 (E4F5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CPSF6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CPSF6 (E4F5F) Rabbit Monoclonal Antibody (CST #92879) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CPSF6 (E4F5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CPSF6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu56 of human CPSF6 protein. Background Cleavage and polyadenylation of pre-mRNA is regulated by a core group of proteins called the cleavage and polyadenylation specificity factors (CPSF) (1,2). The CPSF proteins interact with poly(A) polymerase (PAP) to recognize the AAUAAA sequence motif and add poly(A). CPSF can also interact with downstream cleavage factors to precisely cleave the 3' end of pre-mRNA (2,3). CPSF is brought to 3' ends by the carboxy-terminal domain of the Rpb1 subunit of the RNA Polymerase II complex, where it dissociates and initiates polyadenylation (4). CPSF has been shown to have numerous interactions with viral proteins. The influenza NS1 viral protein binds to CPSF4 to prevent 3' end processing of viral RNAs, inhibiting nuclear export (5). CPSF6 is a 68 kDa member of the CPSF group that has been shown to target the HIV-1 viral capsid (CA) (6). A truncated form of CPSF6, CPSF6-358, localizes exclusively in the cytoplasm and can oligomerize to bind CA, restricting HIV infection and nuclear entry (7,8). Alternate Names CFIM; CFIM68; CFIM72; cleavage and polyadenylation specific factor 6; cleavage and polyadenylation specific factor 6, 68kDa; Cleavage and polyadenylation specificity factor 68 kDa subunit; Cleavage and polyadenylation specificity factor subunit 6; Cleavage factor Im complex 68 kDa subunit; CPSF 68 kDa subunit; CPSF6; HPBRII-4; HPBRII-7; pre-mRNA cleavage factor I, 68kD subunit; pre-mRNA cleavage factor Im (68kD); Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPBRII-4/7 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG