CST, 92991T, Trk (pan) (A7H6R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TrkA/TrkC/TrkB. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Trk (pan) (A7H6R) Rabbit Monoclonal Antibody (CST #92991) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Trk (pan) (A7H6R) Rabbit Monoclonal Antibody detects endogenous levels of total Trk protein. This antibody detects TrkA, TrkB and TrkC. However, the antibody may perferentially detect TrkA over TrkB and TrkB over TrkC. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Tyr791 of human TrkA. Background The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10). Alternate Names BDNF-tropomyosine receptor kinase B; BDNF/NT-3 growth factors receptor; DKFZp781I14186; EIEE58; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; ETV6-NTRK3 fusion partner; gp140trk; GP145-TrkB; GP145-TrkC; gp145(trkC); High affinity nerve growth factor receptor; MTC; neurotrophic receptor tyrosine kinase 1; neurotrophic receptor tyrosine kinase 2; neurotrophic receptor tyrosine kinase 3; Neurotrophic tyrosine kinase receptor type 1; Neurotrophic tyrosine kinase receptor type 2; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 1; neurotrophic tyrosine kinase, receptor, type 2; neurotrophic tyrosine kinase, receptor, type 3; neurotrophin 3 receptor; NT-3 growth factor receptor; NTRK1; NTRK2; NTRK3; OBHD; Oncogene TRK; p140-TrkA; TRK; Trk-A; Trk-B; Trk-C; TRK1; TRK1-transforming tyrosine kinase protein; TRKA; TRKB; TrkB tyrosine kinase; TRKC; TrkC tyrosine kinase; Tropomyosin-related kinase A; Tropomyosin-related kinase B; Tyrosine kinase receptor; tyrosine kinase receptor A; tyrosine kinase receptor B; tyrosine kinase receptor C Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120-140 Source/Isotype: Rabbit IgG