CST, 93026T, DR6 (E8D2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DR6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DR6 (E8D2I) Rabbit Monoclonal Antibody (CST #93026) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DR6 (E8D2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total DR6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His235 of human DR6 protein within the extracellular domain. Background The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases. DR6, also known as TNFRSF21, is a TNFR family member able to induce apoptosis as well as activation of NF-κB and JNK (3). Expression of DR6 is upregulated by NF-κB signaling (4). DR6 appears to play a critical role in the activation and differentiation of T and B lymphocytes (5,6). In the nervous system, β-amyloid precursor protein (APP) activates DR6 to trigger neuronal degeneration (7). Alternate Names BM-018; CD358; Death receptor 6; DR6; MGC31965; TNF receptor superfamily member 21; TNFR-related death receptor 6; TNFRSF21; TNR21; Tumor necrosis factor receptor superfamily member 21; tumor necrosis factor receptor superfamily, member 21 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80, 120 Source/Isotype: Rabbit IgG