CST, 93062S, CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying CD10 mouse. Validated for Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #93062) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:1600 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10/Neprilysin protein. Background CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10 GPR77 carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4). Alternate Names 6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; common acute lymphoblastic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; Mme; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG