CST, 93065S, MTSS1 (D2H4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTSS1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, MTSS1 (D2H4L) Rabbit Monoclonal Antibody (CST #93065) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MTSS1 (D2H4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTSS1 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val650 of human MTSS1 protein. Background Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that identified proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a SHH responsive gene that can help regulate GLI-dependent transcriptional activity (8). Alternate Names DKFZp781P2223; FLJ44694; KIAA0429; metastasis suppressor 1; Metastasis suppressor protein 1; Metastasis suppressor YGL-1; MIM; MIMA; MIMB; missing in metastasis; Missing in metastasis protein; MTSS I-BAR domain containing 1; MTSS1; MTSS1, I-BAR domain containing; Protein MTSS 1 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 95-105 Source/Isotype: Rabbit IgG