CST, 9312S, Tyk2 Antibody

Polyclonal Antibody for studying Tyk2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Tyk2 Antibody (CST #9312) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tyk2 Antibody detects endogenous levels of total Tyk2 protein. No cross-reactivity was detected with other family members at physiological conditions. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding serine 819 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography. Background Tyk2 is a member of the Jak family of protein tyrosine kinases. It associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and IFN-α and β (1-3). Following ligand binding, Tyk2 is activated by phosphorylation of Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-β signaling cascade (5). Alternate Names IMD35; JTK1; Non-receptor tyrosine-protein kinase TYK2; TYK2; tyrosine kinase 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 134 SOURCE: Rabbit