CST, 93169T, CD11b/ITGAM (E4K8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD11b/ITGAM mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD11b/ITGAM (E4K8C) Rabbit Monoclonal Antibody (CST #93169) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 35476 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD11b/ITGAM (E4K8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD11b/ITGAM protein. Non-specific staining was observed in mouse pancreatic acinar cells by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser832 of mouse CD11b/ITGAM protein. Background Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4). Alternate Names CD11 antigen-like family member B; Cd11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; integrin alpha M; Integrin alpha-M; ITAM; Itgam; Leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; Mac-1; Mac-1 alpha; Mac-1a; MAC1; macrophage antigen alpha Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Rabbit IgG