CST, 9321S, Phospho-Tyk2 (Tyr1054/1055) Antibody

Polyclonal Antibody for studying Tyk2 (Tyr1054/Tyr1055) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Tyk2 (Tyr1054/1055) Antibody (CST #9321) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Tyk2 (Tyr1054/1055) Antibody detects endogenous levels of Tyk2 protein only when phosphorylated at Tyr1054/1055. This antibody does not cross-react with Tyk2 protein phosphorylated at other sites. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1054/1055 of human Tyk2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Tyk2 is a member of the Jak family of protein tyrosine kinases. It associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and IFN-α and β (1-3). Following ligand binding, Tyk2 is activated by phosphorylation of Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-β signaling cascade (5). Alternate Names IMD35; JTK1; Non-receptor tyrosine-protein kinase TYK2; TYK2; tyrosine kinase 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 134 SOURCE: Rabbit