CST, 9325S, GSK-3 beta Tag Antibody

Polyclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, GSK-3 beta Tag Antibody (CST #9325) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GSK-3 beta Tag Antibody is optimized to detect transfected levels of GSK-3β-tagged proteins and weakly detects endogenous GSK-3β (46 kDa). Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic GSK-3β-Tag peptide. Antibodies are purified by protein A and peptide affinity chromatography. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only SOURCE: Rabbit