CST, 9331S, Phospho-GSK-3 alpha/beta (Ser21/9) Antibody

Polyclonal Antibody for studying GSK3-alpha (Ser21) phosphate/GSK3-beta (Ser9) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-GSK-3 alpha/beta (Ser21/9) Antibody (CST #9331) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-GSK-3 alpha/beta (Ser21/9) Antibody detects endogenous levels of GSK-3 only when phosphorylated at serine 21 of GSK-3alpha or serine 9 of GSK-3beta. Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to the sequences of human GSK-3 alpha and beta. Antibodies are purified by protein A and peptide affinity chromatography. Background Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Alternate Names DKFZp686D0638; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; GSK3A; GSK3alpha; GSK3B; GSK3beta; GSK3beta isoform; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B Specification REACTIVITY: H M R Mk Z SENSITIVITY: Endogenous MW (kDa): 46 GSK-3beta. 51 GSK-3alpha. SOURCE: Rabbit