CST, 93327S, FTCD (D1R3F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FTCD. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FTCD (D1R3F) Rabbit Monoclonal Antibody (CST #93327) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FTCD (D1R3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total FTCD protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu440 of human FTCD protein. Background Formimidoyltransferase-cyclodeaminase (FTCD) catalyzes two key consecutive reactions in the histidine degradation pathway and links histidine catabolism to one-carbon metabolism. It converts tetrahydrofolate (THF) to 5, 10-methenyl-THF (1,2). Loss of FTCD decreases the sensitivity of cancer cells to chemotherapeutic drug methotrexate, an inhibitor of dihydrofolate reductase (DHFR). DHFR reduces dihydrofolate to tetrahydrofolate, an essential cofactor for nucleotide biosynthesis. Lack of FTCD leads to higher levels of tetrahydrofolate in methotrexate-treated cells, therefore reducing the effect of methotrexate. Conversely, greater depletion of tetrahydrofolate by FTCD through enhanced histidine degradation pathway may boost the efficacy of methotrexate (3,4). Alternate Names formimidoyltetrahydrofolate cyclodeaminase; formimidoyltransferase cyclodeaminase; Formimidoyltransferase-cyclodeaminase; formiminotetrahydrofolate cyclodeaminase; formiminotransferase cyclodeaminase; formiminotransferase-cyclodeaminase; FTCD; glutamate formimidoyltransferase; glutamate formiminotransferase; glutamate formyltransferase; LCHC1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG