CST, 9338S, GSK-3 alpha Antibody

Polyclonal Antibody for studying GSK3-alpha. Validated for Western Blotting. Highly specific and rigorously validated in-house, GSK-3 alpha Antibody (CST #9338) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GSK-3 alpha Antibody detects endogenous levels of total GSK-3alpha protein. This antibody does not cross-react with recombinant GSK-3beta. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly90 of human GSK-3α protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3alpha regulates the production of amyloid-beta peptides, a major component of the plaques that accumulate with progression of Alzheimer's disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-beta production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by gamma secretase, blocking accumulation of amyloid-beta peptides in the brains of mice that overproduce APP (6). Alternate Names DKFZp686D0638; glycogen synthase kinase 3 alpha; Glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK3A; GSK3alpha; Serine/threonine-protein kinase GSK3A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 51 SOURCE: Rabbit