CST, 93400S, SNAP-91/AP180 Antibody

Polyclonal Antibody for studying SNAP-91. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SNAP-91/AP180 Antibody (CST #93400) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SNAP-91/AP180 Antibody recognizes endogenous levels of total SNAP-91/AP180 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp487 of human SNAP-91/AP180 protein. Antibodies are purified by peptide affinity chromatography. Background Clathrin-coated assembly protein SNAP-91, also known as AP180, is an adaptor protein that regulates the endocytosis of proteins and receptors in the membranes at synaptic vesicles (VS), and in the sorting and clustering synaptic vesicle cargo (1,2). SNAP-91/AP180 regulates the formation of clathrin lattices on lipid templates in conjunction with clathrin assembly lymphoid myeloid leukemia (CALM), and often interacts with other endocytic pathways, including the adaptor protein-complex AP-2 (3,4). SNAP-91/AP180 modulates the invagination of the clathrin-coated pit (CCP) by facilitating the clathrin assembly (5). Mutations in the C. elegans SNAP-91/AP180 neuronal homologue unc11 induces large endocytic vesicles and defective neurotransmitter processes (5,6). SNAP-91/AP180 has been associated with and is related to schizophrenia risk variants, resulting in deficits at the pre- and post-synaptic neuronal function (7). In the tau transgenic mouse model of Alzheimer's disease, Tg2576, or the overexpression of SNAP-91/AP180, a selective clathrin-dependent endocytosis of monomeric Aβ peptides results in a greater uptake of Aβ 1-42 compared to Aβ 1-40, increasing the toxicity of cells due to Aβ peptide imbalance (8,9). SNAP-91/AP180 has been proposed as a candidate biomarker for glioblastoma (GBM), along with COL3A1, where a reduction of levels of these proteins was found in patients with GBM and other malignant gliomas (10). Alternate Names 91 kDa synaptosomal-associated protein; AP180; assembly protein, 180kDa; CALM; Clathrin coat assembly protein AP180; Clathrin coat-associated protein AP180; DKFZp781O0519; KIAA0656; Phosphoprotein F1-20; SNAP-91; SNAP91; synaptosomal-associated protein, 91kDa homolog; synaptosomal-associated protein, 91kDa homolog (mouse); synaptosome associated protein 91; synaptosome associated protein 91kDa Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit