CST, 93416S, PAPD7 (F1Q9R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAPD7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PAPD7 (F1Q9R) Rabbit Monoclonal Antibody (CST #93416) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PAPD7 (F1Q9R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAPD7 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His184 of human PAPD7 protein. Background PAP-associated domain-containing protein 5 (PAPD5) and PAPD7, also known as TENT4B and TENT4A, respectively, are poly(A) polymerases involved in a layer of mRNA regulation by depositing guanosine residues on the poly(A) tail. This process, called mixed tailing, interferes with the deadenylase CCR4-NOT complex from efficiently deadenylating the poly(A) tail (1). PAPD5 and PAPD7 form a complex with ZCCHC14 and play a role in stabilization of hepatitis RNA (2-4). Small molecule inhibitors of PAPD5 and PAPD7 are being pursued for treatment against hepatitis B by inhibiting their polyadenylase activity (5,6). Alternate Names DNA polymerase kappa; DNA polymerase sigma; LAK-1; LAK1; Non-canonical poly(A) RNA polymerase PAPD7; PAP associated domain containing 7; PAP-associated domain-containing protein 7; PAPD7; POLK; POLS; poly(A) RNA polymerase D7, non-canonical; polymerase (DNA directed) sigma; polymerase (DNA-directed) sigma; TENT4A; Terminal guanylyltransferase; Terminal nucleotidyltransferase 4A; Terminal uridylyltransferase 5; Topoisomerase-related function protein 4-1; TRAMP-like complex polyadenylate polymerase; TRF4; TRF4-1; TRF41; TUTase 5; TUTASE5 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG