CST, 93473SF, beta-Actin (13E5) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying beta-Actin. Validated for WB,IHC,IF,IF,F,ELISA+. Highly specific and rigorously validated in-house, beta-Actin (13E5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #93473) is ready to ship. Product Usage Information This product is the carrier free version of product #4970. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4970 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity beta-Actin (13E5) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total β-actin protein. Despite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, this antibody does not cross-react with cytoplasmic γ-actin, or any other actin isoforms. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein. Background Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7). Alternate Names ACTB; actin beta; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; BRWS1; I(2)-actin; PS1TP5-binding protein 1; PS1TP5BP1 Specification REACTIVITY: H M R Hm Mk B Pg SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG