CST, 93498T, CD163 (D6U1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD163. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD163 (D6U1J) Rabbit Monoclonal Antibody (CST #93498) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 25121 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD163 (D6U1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD163 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human CD163 protein. Background CD163 is a transmembrane scavenger receptor expressed on the macrophage surface. It has 9 B-type Scavenger Receptor Cysteine-Rich (SRCR) extracellular domains mediating serum haptoglobin clearing/endocytosis, pathogen binding and signal transduction, and calcium binding (1,2). CD163 is used as a surface marker of M2 type macrophages, including M2 type tumor-associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression, and metastasis (3). Inflammatory stimulation and stress signals can induce extracellular domain shedding of CD163 to generate soluble CD163 (sCD163). The increased sCD163 level in serum is associated with low-grade inflammation in disease conditions (4-7). Alternate Names C163A; CD163; CD163 antigen; CD163 molecule; hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; SCARI1; Scavenger receptor cysteine-rich type 1 protein M130; sCD163; soluble CD163 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 160, 170 Source/Isotype: Rabbit IgG