CST, 9349L, Perilipin-1 (D1D8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying perilipin. Validated for WB,IP,IHC,IF,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Perilipin-1 (D1D8) Rabbit Monoclonal Antibody (CST #9349) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57117 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Perilipin-1 (D1D8) Rabbit Monoclonal Antibody detects endogenous levels of total perilipin-1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin-1 protein. Background Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5). Alternate Names FPLD4; Lipid droplet-associated protein; PERI; perilipin 1; Perilipin-1; PLIN; PLIN1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG