CST, 93568S, UNC93B1 (E6C3E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UNC93B1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UNC93B1 (E6C3E) Rabbit Monoclonal Antibody (CST #93568) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UNC93B1 (E6C3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total UNC93B1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr40 of human UNC93B1 protein. Background Signaling by Toll-like receptors (TLRs) is essential for innate and adaptive immune responses (1-3). The endoplasmic reticulum (ER) protein, UNC93B1, was initially identified by a genetic screen analyzing defects in TLR signaling (1,2). UNC93B1 is a multipass transmembrane protein required for normal TLR function and facilitates trafficking of TLRs from the ER to endolysosomes (4,5). UNC93B1 is essential for signaling of TLR3, TLR7, and TLR9 in both humans and mice, and physically interacts with these TLRs in the ER via adaptor protein complex 2 (AP-2) and syntenin-1 (4-7). Mice carrying mutations in UNC93B1 are susceptible to viral infection and autoimmune disease, and mutations in several regions of UNC93B1 in humans have been implicated in autoimmune diseases such as systemic lupus erythematosus, highlighting the role of UNC93B1 in innate and autoimmunity (7-9). Alternate Names hUNC93B1; IIAE1; MGC126617; Protein unc-93 homolog B1; UN93B; unc-93 homolog B1 (C. elegans); unc-93 homolog B1, TLR signaling regulator; unc-93 related protein; Unc-93B1; UNC93; unc93 homolog B; unc93 homolog B1; UNC93B; UNC93B1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG