CST, 93627T, Legumain (D6S4H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LGMN. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Legumain (D6S4H) Rabbit Monoclonal Antibody (CST #93627) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92261 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Legumain (D6S4H) Rabbit Monoclonal Antibody recognizes endogenous levels of total Legumain protein. Multiple bands represent posttranslational modification, including autoproteolytic cleavage, of Legumain. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu251 of human Legumain protein. Background Legumain (LGMN) is an asparaginyl endopeptidase that mediates lysosomal processing of antigen for class II MHC presentation. Under normal conditions, LGMN is mostly found in lysosomes. However, its expression level, cellular compartmentalization, and function may change under certain conditions, including cancer (1, 2, 3). In colorectal cancer, LGMN is upregulated and is found extracellularly and in the nucleus in both primary tumors and colorectal cancer cell lines (4, 5). Legumain forms homodimers and may be activated by posttranslational modifications, including autoproteolytic cleavage, as it moves from one cellular compartment to another (6). Alternate Names AEP; Asparaginyl endopeptidase; cysteine protease 1; Legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25, 35, 55 Source/Isotype: Rabbit IgG