CST, 93638SF, DAP12 (E8P9U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Dap12. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, DAP12 (E8P9U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #93638) is ready to ship. Product Usage Information This product is the carrier free version of product #46088. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 46088 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity DAP12 (E8P9U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total DAP12 protein. Non-specific staining was observed in testis by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro110 of human DAP12 protein. Background DNAX-activating protein 12 (DAP12, TYROBP) is a signaling adaptor for several pathogen receptors expressed by cells of the innate immune system (1). The DAP12 protein structure consists of a short extracellular domain, a transmembrane domain, and a cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM) (2). DAP12 protein is expressed by hematopoietic cells, including NK cells, monocytes, macrophages, dendritic cells, mast cells, basophils, eosinophils, neutrophils, and some γδ T cells and NKT cells (1). DAP12 exists as a homodimer that associates with a variety of receptors involved in pathogen detection, including the KIR family of NK cell receptors (2,3). Ligand binding by DAP12-associated receptors results in phosphorylation of tyrosine residues within the DAP12 ITAM by Src family kinases and leads to activation of Syk or Zap-70 and downstream signaling responses (2). Alternate Names DAP12; DNAX adaptor protein 12; DNAX-activation protein 12; KAR-associated protein; KARAP; killer activating receptor associated protein; Killer-activating receptor-associated protein; PLOSL; PLOSL1; polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy; transmembrane immune signaling adaptor TYROBP; TYOBP; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG