CST, 93667S, TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying ITF2. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #93667) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:400 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4/ITF2 protein. Background Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14). Alternate Names BHLHB19; CDG2T; Class B basic helix-loop-helix protein 19; E2-2; FECD3; Immunoglobulin transcription factor 2; ITF-2; ITF2; MGC149723; MGC149724; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; SL3-3 enhancer factor 2; TCF-4; TCF4; Transcription factor 4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG