CST, 93757S, MAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MADCAM1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody (CST #93757) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 41968 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity MAdCAM-1 (E1V8F) Rabbit Monoclonal Antibody recognizes transfected levels of total MAdCAM-1 protein by western blot and endogenous levels of total MAdCAM-1 protein by immunohistochemistry. The observed molecular weight of 60-85 kDa differs from the predicted molecular weight of 40 kDa due to glycosylation. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAdCAM-1 protein. Background Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is a glycoprotein expressed by endothelial cells at venules of Peyer's patches, intestinal lamina propria, and mesenteric lymph nodes (1). MAdCAM-1 contributes to lymphocyte homing to these tissues, serving as a ligand for α4β7, L-Selectin, and, to a lesser extent, α4β1 (2-6). Expression of MAdCAM-1 is upregulated in patients with inflammatory bowel disease, and blocking MAdCAM-1 reduces colon inflammation by disrupting lymphocyte homing to the intestinal tract (7). In ulcerative colitis, disease activity may be a result of the L-Selectin ligand carbohydrates attached to MAdCAM-1 by -acetylglucosamine-6- -sulfotransferase-1 (GlcNAc6ST-1) rather than the expression levels of MAdCAM-1 alone (4). Alternate Names hMAdCAM-1; MACAM1; MADCA; MAdCAM-1; MADCAM1; Mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1 Specification REACTIVITY: H SENSITIVITY: Endogenous (IHC-P), Transfected (W) MW (kDa): 60-85 Source/Isotype: Rabbit IgG