CST, 93766S, Oxytocin/Neurophysin I (F3X6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OXT. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Oxytocin/Neurophysin I (F3X6H) Rabbit Monoclonal Antibody (CST #93766) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity Oxytocin/Neurophysin I (F3X6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total oxytocin/neurophysin I protein. Non-specific staining was observed in human skeletal myocytes by immunohistochemistry. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp35 of human oxytocin/neurophysin I protein. Background Encoded by the gene, the neuropeptide oxytocin and its carrier protein neurophysin I are synthesized primarily in magnocellular neurons of the hypothalamus. Once synthesized, oxytocin is either secreted in the brain or transported to the neurohypophysis via neurosecretory vesicles, where it is stored and released into the bloodstream (1). Oxytocin acts by binding its lone receptor, OXTR, which in turn binds to Gα(i) or Gα(q) proteins, activating a number of signaling cascades, including mitogen-activated protein kinase (MAPK), protein kinase C (PKC), phospholipase C (PLC), and Ca /calmodulin-dependent protein kinase (CaMK) pathways (1,2). Oxytocin was first described for its important role in parturition and lactation and has since been associated with a number of social behaviors (1,3). Cyclic ADP-ribose hydrolase 1 (CD38) is a key mediator of oxytocin secretion. Genetic polymorphisms related to the oxytocin system, including in the and genes, have been linked to autism spectrum disorder (ASD) (4,5). Oxytocin also has roles in cognitive function and has been proposed as a therapeutic target for Alzheimer's disease (AD) (2). Alternate Names NEU1; Neurophysin 1; neurophysin I; Ocytocin; OT; OT-NPI; OXT; OXT-NPI; Oxytocin; Oxytocin-neurophysin 1; oxytocin-neurophysin I, preproprotein; oxytocin, prepro- (neurophysin I); oxytocin, prepropeptide; oxytocin/neurophysin I prepropeptide Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG