CST, 93790T, EpCAM (E6V8Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EPCAM mouse. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, EpCAM (E6V8Y) Rabbit Monoclonal Antibody (CST #93790) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 IHC Leica Bond: 1:75 - 1:300 Immunohistochemistry (Paraffin): 1:150 - 1:600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 88469 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity EpCAM (E6V8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total EpCAM protein. This antibody is preferred for immunohistochemical analysis of mouse tissues. For human tissue analysis, EpCAM (D9S3P) Rabbit Monoclonal Antibody #14452 is suggested. Species reactivity by immunofluorescence is human only. Species Reactivity: Human, Mouse, Rat, Hamster Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg311 of mouse EpCAM protein. Background Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca -independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3). Alternate Names CD326; EGP; EGP-; EGP-2; Egp31; Egp314; Ep-C; Ep-CAM; EpC; EpCA; Epcam; EpCAM1; Epithelial cell adhesion molecule; epithelial glycoprotein 314; GA733-; GA733-2; gp4; gp40; Ly7; Ly74; lymphocyte antigen 74; mEGP314; panepithelial glycoprotein 314; protein 289A; Tacsd1; Tacst; Tacstd1; Trop-1 protein; TROP1; tumor-associated calcium signal transducer 1 Specification REACTIVITY: H M R Hm SENSITIVITY: Endogenous MW (kDa): 35-45 Source/Isotype: Rabbit IgG