CST, 9380S, Phospho-RanBP3 (Ser58) Antibody

Polyclonal Antibody for studying RANBP3 (Ser126) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-RanBP3 (Ser58) Antibody (CST #9380) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-RanBP3 (Ser58) Antibody recognizes endogenous levels of RanBP3b protein only when phosphorylated at Ser58, and RanBP3a protein only when phosphorylated at corresponding residue Ser126. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser58 of human RanBP3b protein. Antibodies are purified by protein A and peptide affinity chromatography. Background RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with β-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8). Alternate Names DKFZp586I1520; epididymis secretory sperm binding protein; RAN binding protein 3; Ran-binding protein 3; RAN-binding protein-3; RANB3; RANBP3 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit