CST, 93815T, CB1 Receptor (D5N5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CNR1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CB1 Receptor (D5N5C) Rabbit Monoclonal Antibody (CST #93815) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CB1 Receptor (D5N5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total CB1 Receptor protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of mouse CB1 Receptor protein. Background The endocannabinoid system consists of the cannabinoid receptors, CB1 and CB2 receptors, the enzymes that produce and degrade the endogenous cannabinoid ligands (such as FAAH, DAG lipases, and MAG lipase), and the endocannabinoid ligands derived from the metabolism of arachidonic acid, 2-arachidonoylglycerol (2-AG) and anandamide (1-3). CB1 receptor belongs to the superfamily of G protein-coupled receptors (GPCRs) and harbors a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular tail. CB1 receptor is coupled to the Gai/o subunit of the G protein which inhibits adenylyl cyclases and regulates calcium and potassium ion channels (4). CB1 receptor is one of the most abundant GPCRs in the central nervous system. It has been show to play critical roles in the wiring of the brain during development (5), in neuronal plasticity (6), analgesia, drug abuse and metabolic homeostasis (4). In addition, CB1 receptor has been shown to interact with other GPCRs, to give rise to novel pharmacological and signaling heteromers with implication in diseases (7,8). Alternate Names CANN6; Cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB-R; CB1; CB1A; CB1K5; CB1R; central cannabinoid receptor; CNR; CNR1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG