CST, 93882T, CD14 (D1U2Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD14 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CD14 (D1U2Y) Rabbit Monoclonal Antibody (CST #93882) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity CD14 (D1U2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse CD14 protein. This antibody does not cross-react with human CD14 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp327 of mouse CD14 protein. Background CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5). Alternate Names Cd14; CD14 antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 45-52 Source/Isotype: Rabbit IgG