CST, 93908SF, Integrin alpha1/CD49a (E9K2J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ITGA1. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Integrin alpha1/CD49a (E9K2J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #93908) is ready to ship. Product Usage Information This product is the carrier-free version of product #15574. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15574 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Integrin alpha1/CD49a (E9K2J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total integrin α1/CD49a protein. Non-specific staining of mucin was observed by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human integrin α1/CD49a protein. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Integrin α1 (CD49a, VLA-1, ITGA1) pairs with integrin β1 to form an α1β1 dimer on the cell surface. This integrin dimer plays important roles in colorectal cancer and pancreatic cancer cell proliferation, migration, and metastasis (4-6). The cytoplasmic tail of integrin α1 activates ERK and FAK/Src signaling to promote these biological processes (7,8). In immune systems, integrin α1 expression defines a tissue-resident cytotoxic T cell population contributing to both cancer immunity and vitiligo, an autoimmune disease (9,10). Alternate Names CD49 antigen-like family member A; CD49a; Integrin alpha-1; integrin subunit alpha 1; integrin, alpha 1; ITA1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG