CST, 93910S, DDX39A/UAP56 (E3J9O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BAT1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX39A/UAP56 (E3J9O) Rabbit Monoclonal Antibody (CST #93910) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DDX39A/UAP56 (E3J9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX39A and UAP56 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro365 of human UAP56 protein. Background The gene is found in the central MHC region and encodes a member of the DEAD-box family of RNA helicases (1). Also known as DDX39B and BAT1, UAP56 functions as an ATP-dependent splicing factor and RNA helicase in the evolutionary conserved transcription/export (TREX) complex. The TREX complex is recruited to sites of active transcription, where it travels along the length of the gene with RNA polymerase II and exports resulting mRNAs to the cytoplasm (2-8). DDX39, also known as DDX39A, is a DEAD-box helicase highly homologous to UAP56 and is upregulated in lung squamous cell cancers (9,10). DDX39 has been shown to bind to TRF2 to regulate telomere protection (11). Furthermore, DDX39 is overexpressed in several cancer types, which is associated with poor prognosis (12-15). Both UAP56 and DDX39 are hijacked by various viral replication machineries to enable viral reproduction and mRNA export (16-18). UAP56 and DDX39 have also been implicated in promoting the AR-V7 splice variant in advanced prostate cancers (19). Alternate Names 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; BAT1; D6S81E; DDX39B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B; DEAD box protein UAP56; DEAD-box helicase 39B; DEAD-box protein UAP56; DExD-box helicase 39B; DX39B; HLA-B associated transcript 1; HLA-B-associated transcript 1 protein; nuclear RNA helicase (DEAD family); Spliceosome RNA helicase BAT1; Spliceosome RNA helicase DDX39B; UAP56 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG