CST, 9391T, Phospho-Threonine-Proline Mouse Monoclonal Antibody (P-Thr-Pro-101)

Monoclonal Antibody for studying . Validated for Western Blotting,Immunohistochemistry (Paraffin),Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Threonine-Proline Mouse Monoclonal Antibody (P-Thr-Pro-101) (CST #9391) is ready to ship. Product Usage Information Western Blotting: 1:5000 Immunohistochemistry (Paraffin): 1:400 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-Threonine-Proline Mouse Monoclonal Antibody (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. This antibody reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. This antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. This antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides. This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody. Background The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline. As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by western analysis of nocodazole-treated Jurkat cell extracts resolved on 2-D gels. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgM