CST, 93972SF, NeuN (E4M5P) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NeuN. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #93972) is ready to ship. Product Usage Information This product is the carrier free version of product #94403. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 94403 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NeuN (E4M5P) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NeuN protein. This antibody also recognizes a non-specific band of unknown origin at 140 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NeuN protein. Background Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3). Alternate Names FLJ56884; FLJ58356; fox-1 homolog C; Fox-3; FOX3; hCG_1776007; hexaribonucleotide binding protein 3; HRNBP3; NEUN; NeuN antigen; neuronal nuclei; Neuronal nuclei antigen; RBFOX3; RFOX3; RNA binding fox-1 homolog 3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46-55 Source/Isotype: Mouse IgG2a kappa