CST, 93988S, UBQLN4 (E7N4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBQLN4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UBQLN4 (E7N4Q) Rabbit Monoclonal Antibody (CST #93988) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UBQLN4 (E7N4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBQLN4 protein. This antibody cross-reacts with a 150 kDa protein of unknown identity in some lysates and a 95 kDa protein of unknown identity in some rat lysates. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys87 of human UBQLN4 protein. Background Ubiquilin 4 (UBQLN4), a member of the ubiquitin-like (UBL) protein family, mediates protein degradation by binding polyubiquitinated targets as well as the S5a subunit of the proteasome (1,2). UBQLN4 acts as a repressor of homologous recombination-mediated DNA double-strand break repair and drives non-homologous end joining when overexpressed (3). Its role in DNA double-strand break repair is dependent on Ser318, which is phosphorylated by ATM in response to DNA damage (3). UBQLN4 expression is upregulated in colorectal cancer, and high expression is associated with decreased disease-free survival in several other cancers, including neuroblastoma, melanoma, and ovarian cancer (3,4). UBQLN4 has also been shown to directly associate with LC3 and UBQLN1, recruiting UBQLN1 to autophagosomes (5). Alternate Names A1U; A1Up; Ataxin-1 interacting ubiquitin-like protein; ataxin-1 ubiquitin-like interacting protein; Ataxin-1 ubiquitin-like-interacting protein A1U; C1orf6; CIP75; Connexin43-interacting protein of 75 kDa; UBIN; ubiquilin 4; Ubiquilin-4; UBQL4; UBQLN4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG