CST, 93993S, PUS7 (F7V7W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PUS7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PUS7 (F7V7W) Rabbit Monoclonal Antibody (CST #93993) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PUS7 (F7V7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total PUS7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val65 of human PUS7 protein. Background PUS7 is a pseudouridine synthase that can catalyze pseudouridylation of various RNA species (1-3). It is critical for the pseudouridylation of the U2 small nuclear RNA, the branch recognizing portion of the spliceosome, at position 35, which promotes pre-mRNA splicing (2). PUS7 deposits pseudouridine on both mRNAs and snoRNAs, which affects stability (4). Furthermore, during development, PUS7 pseudouridylates tRNA-derived small fragments, activating and targeting them toward the translation initiation complex (5). mutations have been described in intellectual disability and microencephaly (6,7). PUS7 has been implicated in colorectal cancer by binding and stabilizing SirT1, activating Wnt signaling (8). Alternate Names FLJ20485; IDDABS; KIAA1897; MGC17720; pseudouridine synthase 7; pseudouridylate synthase 7 (putative); Pseudouridylate synthase 7 homolog; pseudouridylate synthase 7 homolog (S. cerevisiae); PUS7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG